S2C2 SPA Specimen Preparation Workshop July 13-17, 2026
The Stanford-SLAC CryoEM Center (S2C2) is hosting a focused, hybrid workshop from July 13–17, 2026, for facility users interested in improving cryoEM sample preparation workflows. Designed for researchers with prior cryoEM experience, this program supports those looking to adopt best practices and troubleshoot persistent challenges in grid preparation.
The workshop will include both lectures and practical sessions. Lectures will be broadcast via Zoom and will cover pre-screening sample evaluation, grid selection, freezing instruments, strategies to mitigate air–water interface effects, and emerging vitrification approaches to improve particle distribution and orientation.
The in-person component offers hands-on, interactive, and comparative experience. Participants are encouraged to bring their own samples and directly assess homogeneity, test different preparation approaches, followed by grid screening on the microscopes. Practical sessions will take place in the Biolab in B057 at SLAC. Approved participants must have completed the required access and safety authorization to work in the lab.
Registration is open to S2C2 users, and in-person participation is limited by available space. Registration closes on June 12, 2026, and selected participants will be notified by June 30, 2026.
SPA Specimen Preparation Hybrid Workshop
The Stanford-SLAC CryoEM Center (S2C2) is hosting a focused, hybrid workshop from July 13–17, 2026, for facility users interested in improving cryoEM sample preparation workflows. Designed for researchers with prior cryoEM experience, this program supports those looking to adopt best practices and troubleshoot persistent challenges in grid preparation.The workshop will include both lectures and practical sessions. Lectures will be broadcast via Zoom and will cover pre-screening sample evaluation, grid selection, freezing instruments, strategies to mitigate air–water interface effects, and emerging vitrification approaches to improve particle distribution and orientation.
The in-person component offers hands-on, interactive, and comparative experience. Participants are encouraged to bring their own samples and directly assess homogeneity, test different preparation approaches, followed by grid screening on the microscopes. Practical sessions will take place in the Biolab in B057 at SLAC. Approved participants must have completed the required access and safety authorization to work in the lab.
Registration is open to S2C2 users, and in-person participation is limited by available space. Registration closes on June 12, 2026, and selected participants will be notified by June 30, 2026.
AGENDA
| 9:00 AM - 9:45 AM | Biomolecule characterization with Mass Photometry | Amy Chau, Refeyn |
| 9:45 AM - 10:30 AM | Best practices in preparing cryoEM grids for SPA | Alexandre Cassago, SLAC |
| 10:30 AM - 10:45 AM | Break | |
| 10:45 AM - 11:30 AM | Freezing instruments: Manual Plunger, Vitrobot, Leica, Chameleon, and Vitrojet | Nathan Burrows, SLAC |
| 11:30 AM - 12:15 PM | A microfluidic chip assembly for time-resolved cryo-EM sample preparation | Xiangsong Feng, Columbia University |
| 12:15 PM - 1:30 PM | Lunch | |
| 1:30 PM - 2:15 PM | Application of affinity grids: graphene and streptavidin monolayer | Crystal Stackhouse, Stanford University |
| 2:15 PM - 3:00 PM | Potential mitigation of AWI using DM | Maria Rafiq, Scripps Research |
| 3:00 PM - 4:00 PM | Demonstration of best practices on vitrification, clipping, loading and more | S2C2 Staff |
| -Each participant brings 1-2 specimens and sets of solution per pre-approved proposal -2 grids each condition/sample; 12 condition/samples | |
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| 9:00 AM - 12:00 PM | Two MP |
| 12:00 PM - 1:00 PM | Lunch |
| 1:00 PM - 5:00 PM | Vitrification |
| 9:00 AM - 12:00 PM | Two MP |
| 12:00 PM - 1:00 PM | Lunch |
| 1:00 PM - 5:00 PM | Vitrification |
| -Expert Center Staff Operating Microscopes & Evaluation Grids -Screening Sessions conducted in person and over Zoom -Data will be collected on the best grid with the rest retained for a later microscope session |
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