S2C2 SPA Specimen Preparation Workshop July 13-17, 2026

Alexandra Cassago working on a Sample
Applications are now being accepted.

SPA Specimen Preparation Hybrid Workshop

The Stanford-SLAC CryoEM Center (S2C2) is hosting a focused, hybrid workshop from July 13–17, 2026, for facility users interested in improving cryoEM sample preparation workflows. Designed for researchers with prior cryoEM experience, this program supports those looking to adopt best practices and troubleshoot persistent challenges in grid preparation.

The workshop will include both lectures and practical sessions. Lectures will be broadcast via Zoom and will cover pre-screening sample evaluation, grid selection, freezing instruments, strategies to mitigate air–water interface effects, and emerging vitrification approaches to improve particle distribution and orientation.

The in-person component offers hands-on, interactive, and comparative experience. Participants are encouraged to bring their own samples and directly assess homogeneity, test different preparation approaches, followed by grid screening on the microscopes. Practical sessions will take place in the Biolab in B057 at SLAC. Approved participants must have completed the required access and safety authorization to work in the lab.

Registration is open to S2C2 users, and in-person participation is limited by available space. Registration closes on June 12, 2026, and selected participants will be notified by June 30, 2026.

AGENDA

Day 1, July 13: Lectures (In-Person and via Zoom)
9:00 AM - 9:45 AMBiomolecule characterization with Mass PhotometryAmy Chau,
Refeyn
9:45 AM - 10:30 AMBest practices in preparing cryoEM grids for SPAAlexandre Cassago,
SLAC
10:30 AM - 10:45 AMBreak 
10:45 AM - 11:30 AMFreezing instruments: Manual Plunger, Vitrobot, Leica, Chameleon, and VitrojetNathan Burrows,
SLAC
11:30 AM - 12:15 PMA microfluidic chip assembly for time-resolved cryo-EM sample preparationXiangsong Feng,
Columbia University
12:15 PM - 1:30 PMLunch 
1:30 PM - 2:15 PMApplication of affinity grids: graphene and streptavidin monolayerCrystal Stackhouse,
Stanford University
2:15 PM - 3:00 PMPotential mitigation of AWI using DMMaria Rafiq,
Scripps Research 
3:00 PM - 4:00 PMDemonstration of best practices on vitrification, clipping, loading and moreS2C2 Staff

 

Day 2, July 14: Practical Sessions (Small group of in-person participants)
-Each participant brings 1-2 specimens and sets of solution per pre-approved proposal
-2 grids each condition/sample; 12 condition/samples
9:00 AM - 12:00 PMTwo MP                                                                          
12:00 PM - 1:00 PMLunch
1:00 PM - 5:00 PMVitrification
Day 3, July 15: Practical Sessions (Small group of in-person participants)
9:00 AM - 12:00 PMTwo MP                                                                          
12:00 PM - 1:00 PMLunch
1:00 PM - 5:00 PMVitrification
 
Days 4 & 5, July 16-17: Screening on microscopes + cryoSPARC Live (24-36 grids)
-Expert Center Staff Operating Microscopes & Evaluation Grids
-Screening Sessions conducted in person and over Zoom
-Data will be collected on the best grid with the rest retained for a later microscope session