Publications by Year
These publications have resulted from work performed at the Stanford-SLAC CryoEM Center (S2C2), which is supported by the National Institutes of Health Common Fund Transformative High-Resolution Cryo-Electron Microscopy Program.
| Zhang, M.; Nam, Y.-W; Ramanishka, A.; Xu, Y.; Yasuda, R.M.; Im, D.; Cui, M.; Chandy, G.; Wulff, H.; Structural basis for the subtype-selectivity of KCa2.2 channel activators Nature Portfolio; (Preprint), https://doi.org/10.21203/rs.3.rs-6568445/v1 |
| Mietzsch, M.; Hsi, J.; Nelson, A.R.; Khandekar, N.; Huang, A.-M.; Smith, N.J.C.; Zachary, J.; Potts, L.; Farrar, M.A.; Chipman, P.; Ghanem, M.; Alexander, I.E.; Logan, G.J.; Huiskonen, J.T.; McKenna, R.; Structural characterization of antibody-responses following Zolgensma treatment for AAV capsid engineering to expand patient cohorts. Nature Communications; 19 April 2025 16, 1, 3731 https://doi.org/10.1038/s41467-025-59088-4 |
| Nam, Y.-W.; Im, D.; Garcia, A.S.C.; Tringides, M.L.; Nguyen, H.M.; Liu, Y.; Orfali, R.; Ramanishka, A.; Pintilie, G.; Su, C.-C.; Cui, M.; Logothetis, D.E.; Yu, E.W.; Wulff, H.; Chandy, K.G.; Zhang, M.; Cryo-EM structures of the small-conductance Ca2+-activated KCa2.2 channel. Nature Communications; 17 April 2025 16, 3690 https://doi.org/10.1038/s41467-025-59061-1 |
| Varanese, L.; Xu, L.; Peters. C.E.; Pintilie, G.; Roberts, D.S.; Raj, S.; Liu, M.; Ooi, Y.S.; Diep, J.; Qiao, W.; Richards, C.M.; Callaway, J.; Bertozzi, C.R.; Jabs, S.; de Vries, E.; van Kuppeveld, F.J.M.; Nagamine, C.M; Chiu, W.; Carette, J.E.; MFSD6 is an entry receptor for enterovirus D68. Nature; 25 March 2025 https://doi.org/10.1038/s41586-025-08908-0 |
| Nayak, A.R.; Sokolova, V.; Sillamaa, S.; Herbine, K.; Sedman, J.; Temiakov, D.; Structural basis for intrinsic strand displacement activity of mitochondrial DNA polymerase. Nature Communications 11 March 2025 16(2417) https://doi.org/10.1038/s41467-025-57594-z |
| He, Z.; Zhang, J.; Xu, Y.; Fine, E.J.; Suomivuori, C.-M.; Dror, R.O.; Feng, L.; Structure of mitochondrial pyruvate carrier and its inhibition mechanism. Nature; 5 March 2025 https://doi.org/10.1038/s41586-025-08667-y |
| Rubio, A.A.; Baharani, V.A.; Dadonaite, B.; Parada, M.; Abernathy, M.E.; Wang, Z.; Lee, Y.E.; Eso, M.R.; Jennie, P.; Ramos, I.; Chen, T.; El Nesr, G.; Bloom, J.D.; Bieniasz, P.D.; Nussenzweig, M.C.; Barnes, C.O.; Bispecific antibodies targeting the N-terminal and receptor binding domains potently neutralize SARS-CoV-2 variants of concern. Science Translational Medicine; 5 March 2025 17(788) doi: 10.1126/scitranslmed.adq5720 |
| Wang, K.; Li, L.; Kenny, S.; Gan, D.; Reistma, J.M.; Zhou, Y.; Das, C.; Liu, X.; Molecular mechanisms of CAND2 in regulating SCF ubiquitin ligases. Nature Communications; 26 February 25 1998(16)1 DOI: https://doi.org/10.1038/s41467-025-57065-5 |
| Zhang, Z.; Chiang, H.T.; Xia, Y.; Avakyan, N.; Sonani, R.R.; Wang, F.; Egelman, E.H.; De Yoreo, J.J.; Pozzo, L.D.; Tezcan, F.A.; Design of light- and chemically responsive protein assemblies through host- guest interactions. Chem; 10 February 2025 https://doi.org/10.1016/j.chempr.2024.102407 |
| Gliwa, K.; Hull, J.; Kansol, A.; Zembruski, V.; Lakshmanan, R.; Mietzsch, M.; Chipman, P.; Bennett, A.; McKenna, R.; Biophysical and structural insights into AAV genome ejection. Journal of Virology; 5 February 2025 Biophysical and structural insights into AAV genome ejection |
| Zhao, Y.; Vidossich, P.; Forbush, B.; Ma, J.; Rinehart, J.; De Vivo, M.; Cao, E.; Structural basis for human NKCC1 inhibition by loop diuretic drugs. EMBO Journal; 28 January 2025 DOI: 10.1038/s44318-025-00368-6 |
| Kretsch, R.C.; Li, S.; Pintilie, G.; Palo, M.Z.; Case, D.A.; Das, R.; Zhang,K.; Chiu,W.; Complex Water Networks Visualized through 2.2-2.3 Å Cryogenic Electron Microscopy of RNA. bioRxiv (Preprint) 24 January 2025 doi: https://doi.org/10.1101/2025.01.23.634578 |
| Li, Y.; Zhu, J.; Zhang, Z.; Wei, J.; Wang, F.; Meisl, G.; Knowles, T.P.J.; Egelman, E.H.; Tezcan, A.; Transforming an ATP-dependent enzyme into a dissipative, self-assembling system. Nature Chemical Biology 13 January 2025 1552-4469; https://doi.org/10.1038/s41589-024-01811-1 |
| Ganguly, C.; Martin, L.; Aribam, S.; Thomas, L.M.; Rajan, R.; Helical transition of the bridge helix of Cas12a is an allosteric regulator of R-loop formation and RuvC activation. bioRxiv (Preprint) 10 January 2025 https://www.biorxiv.org/content/10.1101/2025.01.09.632262v1 |
| Jabbari, K.; Mietzach, M.; Hsi, J.; Chipman, P.; Qiu, J.; McKenna, R.; The Structural, Biophysical, and Antigenic Characterization of the Goose Parvovirus Capsid. Microorganisms 3 January 2025 13(1),80; https://doi.org/10.3390/microorganisms13010080 |
| Deng, X.; Sun, L.; Zhang, M.; Basavaraj, R.; Wang, J.; Weng, Y.-L.; Gao, Y.; Biochemical Profiling and Structural Basis of ADAR1-Mediated RNA Editing. bioRxiv (Preprint) 2 January 2025 |
| de March, C.A.; Ma, N.; Billesbolle, C.B.; Tewari, J.; del Torrent, C.L.; van der Velden, W.J.C.; Ojiro, I.; Takayama, I.; Faust, B.; Li, L.; Vaidehi, N.; Manglik, A.; Matsunami, H.; Engineered odorant receptors illuminate the basis of odour discrimination. Nature 30 October 2024 DOI: https://doi.org/10.1038/s41586-024-08126-0 |
| Nguyen, B.A.; Afrin, S.; Yakubovska, A.; Singh, V.; Pedretti, R.; Bassett, P.; Pekala, M; Alicea, J.V.; Kunach, P.; Wang, Lanie; Lemoff, A.; Kluve-Beckerman, B.; Saelices, L.; ATTRv-V30M amyloid fibrils from heart and nerves exhibit structural homogeneity. Structure 17 October 2024 https://doi.org/10.1016/j.str.2024.09.021 |
| Cogan, D.P.; Soohoo, A.M.; Chen, M.; Liu, Y.; Brodsky, K.L.; Khosla, Chaitan; Structural basis for intermodular communication in assembly-line polyketide biosynthesis. Nature Chemical Biology 23 August 2023 DOI: https://doi.org/10.1038/s41589-024-01709-y |
| Zhao, Y.; Schubert, H.; Blakely, A.; Forbush, B.; Smith, M.D.; Rinehart, J.; Cao, E.; Structural bases for Na+-Cl− cotransporter inhibition by thiazide diuretic drugs and activation by kinases. Nature Communications 14 August 2024 15(1)7006 DOI: https://doi.org/10.1038/s41467-024-51381-y |
| Held, R.G.; Liang, J.; Brunger, A.T.; Nanoscale architecture of synaptic vesicles and scaffolding complexes revealed by cryo-electron tomography. Neuroscience 26 June 2024 121(27) DOI: https://doi.org/10.1073/pnas.2403136121 |
| Chien, C.-T.; Puhl, H.; Vogel, S.S.; Molloy, J.E.; Chiu, W.; Khan, S.; Hub stability in the calcium calmodulin-dependent protein kinase II. Communications Biology 25 June 2024 7(766) DOI: https://doi.org/10.1038/s42003-024-06423-y |
| Suryadevara, N.; Otrelo-Cardoso, A.R.; Kose, N.; Hu, Y.-X.; Binshtein, E.; Wolters, R.M.; Greninger, A.L.; Handal, L.S.; Carnahan, R.H.; Moscona, A.; Jardetsky, T.S.; Crowe, Jr., J.E.: Functional and structural basis of human parainfluenza virus type 3 neutralization with human monoclonal antibodies. Nature Microbiology 10 June 2024 DOI: https://doi.org/10.1038/s41564-024-01722-w |
| Park, J.; Herrmann, G.K.; Roy, A.; Shumate, C.K.; Cisneros, G.A.; Yin, Y.W.; An interaction network in the polymerase active site is a prerequisite for Watson-Crick base pairing in Pol γ. Science Advances 24 May 2024, 10(21) DOI: 10.1126/sciadv.adl3214 |
| Eisenberg, D.; Hou, K.; Ge, P.; Sawaya, M.; Dolinsky, J.; Yang, Y.; Jiang, Y.X.; Lutter, L.; Boyer, D.; Cheng, X.; Pi, J.; Zhang, J.; Lu, J.; Yang, S.; Yu, Z.; Feigon, J.; How short peptides can disassemble ultra-stable tau fibrils extracted from Alzheimer’s disease brain by a strain-relief mechanism. Nature Portfolio (Preprint 05.08.24 ) DOI: https://doi.org/10.21203/rs.3.rs-4152095/v1 |
| Chew, B.L.A.; Ngoh, A.Q.; Phoo, W.W.; Weng, M.J.G.; Shen, H.J.; Chan, K.W.K.; Tan, E.Y.J.; Gelbart, T.; Xu, C.; Tan, G.S.; Vasudevan, S.G.; Luo, D.; Structural basis of Zika virus NS1 multimerization and human antibody recognition. NPJ Viruses 25 April 2024, 2(1) https://doi.org/10.1038/s44298-024-00024-6 page 14 |
| Thongchol, J.; Yu, Z.; Harb, L.; Lin, Y.; Koch, M.; Theodore, M.; Narsaria, U.; Shaevitz, J.; Gitai, Z.; Wu, Y.; Zhang, J.; Zeng, L.; Removal of Pseudomonas type IV pili by a small RNA virus. Science 5 April 2024, 384(6691) https://doi.org/10.1126/science.adl0635 |
| Balana, A.T.; Mahul-Mellier, A.-L.; Nguyen, B.A.; Horvath, M.; Javed, A.; Hard, E.R.; Jasiqi, Y.; Singh, P.; Afrin, S.; Pedretti, R.; Singh, V.; Lee, V.M.-Y.; Luk, K.C.; Saelices, L.; Lashuel H.A.; Pratt, M.R.; O-GlcNAc forces an α-synuclein amyloid strain with notably diminished seeding and pathology. Nature Chemical Biology 12 February 2024, https://doi.org/10.1038/s41589-024-01551-2 |
| Cookis, T.; Lydecker, A.; Sauer, P.; Kasinath, V.; Nogales, E.; Structural basis for the inhibition of PRC2 by active transcription histone posttranslational modifications. bioRxiv preprint 10 February 2024, https://doi.org/10.1101/2024.02.09.579730 |
| Sharma, S.; Luo, M.; Patel, H.; Mueller, D.M.; Liao, M.; Conformational ensemble of yeast ATP synthase at low pH reveals unique intermediates and plasticity in F1–Fo coupling. Nature Structural & Molecular Biology 5 February 2024, 1545-9985 https://doi.org/10.1038/s41594-024-01219-4 |
| Masse, M.M.; Hutchinson, R.B.; Morgan, C.E.; Allaman, H.J.; Guan, H.; Yu, E.W.; Cavagnero, S.; Mapping Protein–Protein Interactions at Birth: Single-Particle Cryo-EM Analysis of a Ribosome–Nascent Globin Complex. ASC Cent. Sci. XXXX, XXX, XXX-XXX 1 February 2024; https://doi.org/10.1021/acscentsci.3c00777 |
| Nguyen, B.A.; Singh, V.; Afrin, S.; Yakubovska, A.; Wang, L.; Ahmed, Y.; Pedretti, R.; Fernandez-Ramirez, M.D.C.; Sing, P.; Pekala, M. Cabrera Hernandez, L.O.; Kumar, S.; Lemoff, A.; Gonzalez-Prieto, R.; Sawaya, M.R.; Eisenberg, D.S.; Benson, M.D.; Saelices, L.; Structural polymorphism of amyloid fibrils in ATTR amyloidosis revealed by cryo-electron microscopy. Nature Communications | 17 January 2024; 15, 581; https://doi.org/10.1038/s41467-024-44820-3 |
| Yang, R.; Ko, Y.-H.; Li, F.; Lokareddy, R.K.; Hou, C.-F. D.; Kim, C.; Klein, S.; Antolinez, S.; Marin, J.F.; Perez-Segura, C.; Jarrold, M.F.; Zlotnick, A.; Hadden-Perilla, J.A.; Cingolani, G.; Structural basis for nuclear import of hepatitis B virus (HBV) nucleocapsid core. Science Advances 10 January 2024; 10:2; DOI:10.1126/sciadv.adi7606 |
| Wang, L.; Hoang, A.; Gil-lturbe, E.; Laganowsky, A.; Quick, M.; Zhou, M.; Mechanism of anion exchange and small-molecule inhibition of pendrin. Nature Communications 01 January 2024; 15:346; https://doi.org/10.1038/s41467-023-44612-1 |
| Shen, Z.; Lin, Q.; Yang, X-Y.; Fosuah, E.; Fu, T-M.; Assembly-mediated activation of the SIR2-HerA supramolecular complex for anti-phage defense. Molecular Cell 21 December 2023; 83, 24; https://doi.org/10.1016/j.molcel.2023.11.007 |
| Oh, J.; Shan, Z.; Hoshika, S.; Xu, J.; Chong, J.; Benner, S.A.; Lyumkis, D.; Wang, D.; A unified Watson-Crick geometry drives transcription of six-letter expanded DNA alphabets by E. coli RNA polymerase. Nature Communications 12 December 2023; 14,8219; https://doi.org/10.1038/s41467-023-43735-9 |
| Rosenberg, G.M.; Abskharon, R.; Boyer, D.R.; Ge, P.; Sawaya, M.R.; Eisenberg, D.S.; Fibril structures of TFG Protein mutants validate identification of TFG as a disease-related amyloid protein by the IMPAcT method. PNAS Nexus 20 November 2023; pgad402; https://doi.org/10.1093/pnasnexus/pgad402 |
| Liu, Q.; Stadtmueller, B.M.; SIgA structures bound to Streptococcus pyogenes M4 and human CD89 provide insights into host-pathogen interactions. Nature Communications 23 October 2023; https://doi.org/10.1038/s41467-023-42469-y |
| Gangwar, S.P.;Yen, L.Y.; Yelshanskaya, M.V., Korman, A.; Jones, D.R.; Sobolevsky A.I.; Modulation of GluA2-γ5 synaptic complex desensitization, polyamine block and antiepileptic perampanel inhibition by auxiliary subunit cornichon-2. Natural Structural & Molecular Biology 31, August 2023, https://doi.org/10.1038/s41594-023-01080-x |
| Velez, M.; Mietzsch, M.; Hsi, J.; Bell, L.; Chipman, P.; Fu, X.; McKenna, R.; Structural Characterization of Canine Minute Virus, Rat, and Porcine Bocavirus. Viruses, 24 August 2023, 15(9), 1799; https://doi.org/10.3390/v15091799 |
| Logan, G.J.; Mietzsch, M.; Khandekar, N.; D'Silva, A.; Anderson, D.; Mandwie, M.; Hsi, J.; Nelson, A.R.; Chipman, P.; Jackson, J.; Schofield, P.; Christ, D.; Goodnow, C.C.; Reed, J.H.; Farrar, M.A.; McKenna, R.; Alexander, I.E.; Structural and functional characterization of capsid binding by anti-AAV9 monoclonal antibodies from infants after SMA gene therapy. Molecular Therapy, 2023, doi: https://doi.org/10.1016/j.ymthe.2023.03.032 |
| Zimanyi, C.M.; Rayaprolu, V.; Keeping up with Current Practices in a Rapidly Evolving Field Through Dissemination of "Tips and Tricks" in a Monthly Webinar. Microscopy Today, 31 May 2023, Volume 31, Issue 3, Pages 28-33, https://doi.org/10.1093/mictod/qaad024 |
| Park, J.; Herrmann, G.K.; Mitchell, P.G.; Sherman, M.B.; Yin, Y.W.; Poly coordinates DNA synthesis and proofreading to ensure mitochondrial genome integrity. Nature Structural & Molecular Biology, 18 May 2023, doi: https://doi.org/10.1038/s41594-023-00980-2 |
| Chen, Z.; Monda, A.; Ali, F.A.; Jang, S.; Niranjan, S.; Montaño, J.L.; Zaro, B.W.; Minor, D.L.; EMC chaperone-Cav structure reveals an ion channel assembly intermediate. Nature 17 May 2023, doi: https//doi.org/10.1038/s41586-023-06175-5 |
| Gupta, A.M.; Lentzsch, A.M.; Siegel, A.; Yu, Z.; Chio, U.S.; Cheng, Y.; Shan, S.-O.; Dodecamer assembly of a metazoan AAA+ chaperone couples substrate extraction to refolding. Science Advances, 10 May 2023, 9(19), DOI: 10.1126/sciadv.adf5336 |
| Weidenbacher, P. A.-B.; Sanyal, M.; Friedland, N.; Tang, S.; Arunachalam, P.S.; Hu, M.; Kumru, O.S.; Morris, M.K.; Fontenot, J.; Shirreff, L.; Do, J.; Cheng, Y.-C.; Vasudevan, G.; Feinberg, M.B.; Villinger, F.J.; Hanson, C.; Joshi, S.B.; Volkin, D. B.; Pulendran, B.; Kim, P.S.; A ferritin-based COVID-19 nanoparticle vaccine that elicits robust, durable, broad-spectrum neutralizing antisera in non-human primates. Nature Communications, 17 April 2023, 14, 2149, https://doi.org/10.1038/s41467-023-37417-9 |
| Guo, X.; Zhang, J.; Han, L.; Lee, J.; Williams, S.C.; Forsberg, A.; Xu, Y.; Narehood Austin, R.; Feng, L.; Structure and mechanism of the alkane-oxidizing enzyme AlkB. Nature Communications, 17 April 2023, 14, 2180, https://doi.org/10.1038/s41467-023-37869-z |
| Yang, K.; Wang, C.; Kreutzberger, A.J.B.; White, K.I.; Pfuetzner, R.A.; Esquivies, L.; Kirchhausen, T.; Brunger, A.T. Structure- based design of a SARS-CoV-2 Omicron-specific inhibitor. Proceedings of the National Academy of Sciences, 2023.03.20, 120(13), e2300360120. https://doi.org/10.1073/pnas.2300360120 |
| Gangwar, S.P.; Yen, L.Y.; Yelshanskaya, M.V.; Sobolevsky, A.I. Positive and negative allosteric modulation of GluK2 kainate receptors by BPAM344 and antiepileptic perampanel. Cell Reports, 2023.02.28, 42(2), 112124, https//doi.org/10.106/j.cerep.2023.112124 |
| Fan, M.; Zhang, J.; Lee, C-L.; Zhang, J.; Feng, L. Structure and thiazide inhibition mechanism of the human Na-CI cotransporter. Nature, 2023.02.15, 614, 788-793 (2023), https://doi.org/10.1038/s41586-023-05718-0 |
| Richards, L.S.; Flores, M.D.; Zink, S.; Schibrowsky, N.A.; Sawaya, M.R.; Rodriguez, J.A. Cryo-EM Structure of a Human LECT2 Amyloid Fibril Reveals a Network of Polar Ladders at its Core. bioRxiv (Preprint 02.11.23) https://doi.org/10.1101/2023.02.08.527771 |
| Molodtsov, V.; Wang, C.; Firlar, E.; Kaelber, J.T.; Ebright, R.H. Structural basis of Rho-dependent transcription termination. Nature 25 January 2023, 614, 367-374, https://doi.org/10.1038/s41586-022-05658-1 |
| Shen, J; Wilbon, A.S.; Zhou, M.; Pan, Y. Mechanism of Ca2+ transport by ferroportin. eLife, 2023.01.23, 12:e82947, https://doi.org/10.7554/eLife.82947 |
| Nguyen, B.A.; Afrin, S.; Singh, V.; Ahmed, Y.; Pedretti, R.; Fernandez-Ramirez, M.D.C.; Benson, M.D.; Sawaya, M.; Singh, P.; Cao, Q.; Boyer, D.; Pope, A.; Wydorski, P.; Kumar, Si.; Chhapra, F.; Eisenberg, D.; Saelices, L.; Structural polymorphism of amyloid fibrils in cardiac transthyretin amyloidosis revealed by cryo-electron microscopy. bioRxiv (Preprint Posted 10.12.22) https://doi.org/10.1101/2022.06.21.496949 |
| Li, S.; Palo, M.Z.; Pintilie, G.; Zhang, X.; Su, Z.; Kappel, K.; Chiu, W.; Zhang, K.; Das, R. Topological crossing in the misfolded tetrahymena ribozyme resolved by cryo-EM. Proc national Acad Sci. 2022, 119, e2209146119. https//doi.org/10.1073/pnas.2209146119 |
| Yu, Z.; Chen, J.; Takagi, E.; Wang, F.; Saha, B.; Liu, X.; Joubert, L.-M.; Gleason, C. E.; Jin, M.; Li, C.; Nowotny, C.; Agard, D.; Cheng, Y.; Pearce, D. Interactions between MTORC2 Core Subunits Rictor and MSin1 Dictate Selective and Context-Dependent Phosphorylation of Substrate Kinases SGK1 and Akt. J Biol Chem 2022, 102288. https://doi.org/10.1016/j.jbc.2022.102288 |
| Smith, E. M.; Ferrell, S.; Tokars, V. L.; Mondragón, A. Structures of an Active Type III-A CRISPR Effector Complex. Structure 2022, 30 (8), 1109-1128.e6. https://doi.org/10.1016/j.str.2022.05.013 |
| Khant, H.; Li, Y. T.; Shankar, M.; Hecksel, C.; Mitchell, P.; Liu, Y.; Joubert, L.-M.; Zhang, C.; Dunn, L.; Schmid, M.; Hedman, B.; Chiu, W. Cryo-EM Information Management System and Sample Evaluation at Stanford-SLAC Cryo-EM Center. Microsc Microanal 2022, 28 (S1), 1304–1305. https://doi.org/10.1017/s1431927622005359 |
| Rutledge, H. L.; Cook, B. D.; Nguyen, H. P. M.; HerzikJr., M. A.; Tezcan, F. A. Structures of the Nitrogenase Complex Prepared under Catalytic Turnover Conditions. Science 2022, eabq7641. https://doi.org/10.1126/science.abq7641 |
| Li, F.; Eriksen, J.; Oses-Prieto, J.A.; Gomez, Y.K.; Xu, H.; Finer-Moore, J.; Nguyen, P.; Bowen, A.; Nelson, A.; Burlingame, A.; Grabe, M.; Stroud, R.M.; Edwards, R.H. Allosteric Regulation of a Synaptic Vesicle Glutamate Transporter. BioRxiv2022, 2022.07.26.501550. https://doi.org/10.1101/2022.07.26.501550 (preprint) |
| Kim, D. S.; Watkins, A.; Bidstrup, E.; Lee, J.; Topkar, V.; Kofman, C.; Schwarz, K. J.; Liu, Y.; Pintilie, G.; Roney, E.; Das, R.; Jewett, M. C. Three-Dimensional Structure-Guided Evolution of a Ribosome with Tethered Subunits. Nat Chem Biol 2022, 1–9. https://doi.org/10.1038/s41589-022-01064-w |
| Fontana, P.; Dong, Y.; Pi, X.; Tong, A. B.; Hecksel, C. W.; Wang, L.; Fu, T.-M.; Bustamante, C.; Wu, H. Structure of Cytoplasmic Ring of Nuclear Pore Complex by Integrative Cryo-EM and AlphaFold. Science 2022, 376 (6598), eabm9326. https://doi.org/10.1126/science.abm9326 |
| Yelshanskaya, M. V.; Patel, D. S.; Kottke, C. M.; Kurnikova, M. G.; Sobolevsky, A. I. Opening of Glutamate Receptor Channel to Subconductance Levels. Nature 2022, 605 (7908), 172–178. https://doi.org/10.1038/s41586-022-04637-w |
| Yang, K.; Wang, C.; White, K. I.; Pfuetzner, R. A.; Esquivies, L.; Brunger, A. T. Structural Conservation among Variants of the SARS-CoV-2 Spike Postfusion Bundle. Proc National Acad Sci 2022, 119 (16), e2119467119. https://doi.org/10.1073/pnas.2119467119 |
| Abskharon, R.; Sawaya, M. R.; Boyer, D. R.; Cao, Q.; Nguyen, B. A.; Cascio, D.; Eisenberg, D. S. Cryo-EM Structure of RNA-Induced Tau Fibrils Reveals a Small C-Terminal Core That May Nucleate Fibril Formation. Proc National Acad Sci 2022, 119 (15), e2119952119. https://doi.org/10.1073/pnas.2119952119 |
| Fan, C.; Rees, D. C. Glutathione Binding to the Plant AtAtm3 Transporter and Implications for the Conformational Coupling of ABC Transporters. Elife 2022, 11, e76140. https://doi.org/10.7554/elife.76140 |
| Jiang, M.; Shin, J.; Simeon, R.; Chang, J.-Y.; Meng, R.; Wang, Y.; Shinde, O.; Li, P.; Chen, Z.; Zhang, J. Structural Dynamics of Receptor Recognition and PH-Induced Dissociation of Full-Length Clostridioides Difficile Toxin B. Plos Biol 2022, 20 (3), e3001589. https://doi.org/10.1371/journal.pbio.3001589 |
| Kafai, N. M.; Williamson, L. E.; Binshtein, E.; Sukupolvi-Petty, S.; Gardner, C. L.; Liu, J.; Mackin, S.; Kim, A. S.; Kose, N.; Carnahan, R. H.; Jung, A.; Droit, L.; Reed, D. S.; Handley, S. A.; Klimstra, W. B.; Crowe, J. E.; Diamond, M. S. Neutralizing Antibodies Protect Mice against Venezuelan Equine Encephalitis Virus Aerosol Challenge. J Exp Med 2022, 219 (4), e20212532. https://doi.org/10.1084/jem.20212532 |
| Koo, C. W.; Tucci, F. J.; He, Y.; Rosenzweig, A. C. Recovery of Particulate Methane Monooxygenase Structure and Activity in a Lipid Bilayer. Science 2022, 375 (6586), 1287–1291. https://doi.org/10.1126/science.abm3282 |
| Tsai, K.; Stojković, V.; Lee, D. J.; Young, I. D.; Szal, T.; Klepacki, D.; Vázquez-Laslop, N.; Mankin, A. S.; Fraser, J. S.; Fujimori, D. G. Structural Basis for Context-Specific Inhibition of Translation by Oxazolidinone Antibiotics. Nat Struct Mol Biol 2022, 29 (2), 162–171. https://doi.org/10.1038/s41594-022-00723-9 |
| Zimanyi, C. M.; Kopylov, M.; Potter, C. S.; Carragher, B.; Eng, E. T. Broadening Access to CryoEM through Centralized Facilities. Trends Biochem Sci 2021, 47 (2), 106–116. https://doi.org/10.1016/j.tibs.2021.10.007 |
| Zhou, W.; Bammes, B.; Mitchell, P. G.; Betz, K.; Chiu, W. Electron Crystallography of Chiral and Non-Chiral Small Molecules. Ultramicroscopy 2022, 232, 113417. https://doi.org/10.1016/j.ultramic.2021.113417 |
| Klykov, O.; Gangwar, S. P.; Yelshanskaya, M. V.; Yen, L.; Sobolevsky, A. I. Structure and Desensitization of AMPA Receptor Complexes with Type II TARP Γ5 and GSG1L. Mol Cell 2021, 81 (23), 4771-4783.e7. https://doi.org/10.1016/j.molcel.2021.09.030 |
| Cogan, D. P.; Zhang, K.; Li, X.; Li, S.; Pintilie, G. D.; Roh, S.-H.; Craik, C. S.; Chiu, W.; Khosla, C. Mapping the Catalytic Conformations of an Assembly- Line Polyketide Synthase Module. Science 2021, 374 (6568), 729–734. https://doi.org/10.1126/science.abi8358 |
| Neuberger, A.; Nadezhdin, K. D.; Sobolevsky, A. I. Structural Mechanisms of TRPV6 Inhibition by Ruthenium Red and Econazole. Nat Commun 2021, 12 (1), 6284. https://doi.org/10.1038/s41467-021-26608-x |
| Harris, J. A.; Faust, B.; Gondin, A. B.; Dämgen, M. A.; Suomivuori, C.-M.; Veldhuis, N. A.; Cheng, Y.; Dror, R. O.; Thal, D. M.; Manglik, A. Selective G Protein Signaling Driven by Substance P–Neurokinin Receptor Dynamics. Nat Chem Biol 2022, 18 (1), 109–115. https://doi.org/10.1038/s41589-021-00890-8 |
| Sheedlo, M. J.; Durie, C. L.; Chung, J. M.; Chang, L.; Roberts, J.; Swanson, M.; Lacy, D. B.; Ohi, M. D. Cryo-EM Reveals New Species-Specific Proteins and Symmetry Elements in the Legionella Pneumophila Dot/Icm T4SS. Elife 2021, 10, e70427. https://doi.org/10.7554/elife.70427 |
| Zhang, K.; Zheludev, I. N.; Hagey, R. J.; Haslecker, R.; Hou, Y. J.; Kretsch, R.; Pintilie, G. D.; Rangan, R.; Kladwang, W.; Li, S.; Wu, M. T.-P.; Pham, E. A.; Bernardin-Souibgui, C.; Baric, R. S.; Sheahan, T. P.; D’Souza, V.; Glenn, J. S.; Chiu, W.; Das, R. Cryo-EM and Antisense Targeting of the 28-KDa Frameshift Stimulation Element from the SARS-CoV-2 RNA Genome. Nat Struct Mol Biol 2021, 28 (9), 747–754. https://doi.org/10.1038/s41594-021-00653-y |
| Hecksel, C.; Zhang, K.; Pintilie, G.; Mitchell, P.; Li, Y.-T.; Chiu, W. High Resolution Data Collection at S2C2, a National CryoEM Center. Microsc Microanal 2021, 27 (S1), 1152–1154. https://doi.org/10.1017/s1431927621004359 |
| Eriksen, J.; Li, F.; Stroud, R. M.; Edwards, R. H. Allosteric Inhibition of a Vesicular Glutamate Transporter by an Isoform-Specific Antibody. Biochemistry-us 2021, 60 (32), 2463–2470. https://doi.org/10.1021/acs.biochem.1c00375 |
| Hu, W.; Parkinson, C.; Zheng, H. Mechanistic Insights Revealed by YbtPQ in the Occluded State. BioRxiv 2021, 2021.06.15.448544. https://doi.org/10.1101/2021.06.15.448544 (preprint) |
| He, Y.; Wang, Y.; Liu, B.; Helmling, C.; Sušac, L.; Cheng, R.; Zhou, Z. H.; Feigon, J. Structures of Telomerase at Several Steps of Telomere Repeat Synthesis. Nature 2021, 593 (7859), 454–459. https://doi.org/10.1038/s41586-021-03529-9. |
| Horikoshi, N.; Hwang, S.; Gati, C.; Matsui, T.; Castillo-Orellana, C.; Raub, A. G.; Garcia, A. A.; Jabbarpour, F.; Batyuk, A.; Broweleit, J.; Xiang, X.; Chiang, A.; Broweleit, R.; Vöhringer-Martinez, E.; Mochly-Rosen, D.; Wakatsuki, S. Long-Range Structural Defects by Pathogenic Mutations in Most Severe Glucose-6-Phosphate Dehydrogenase Deficiency. Proc National Acad Sci 2021, 118 (4), e2022790118. https://doi.org/10.1073/pnas.2022790118 |
| Powell, A. E.; Zhang, K.; Sanyal, M.; Tang, S.; Weidenbacher, P. A.; Li, S.; Pham, T. D.; Pak, J. E.; Chiu, W.; Kim, P. S. A Single Immunization with Spike- Functionalized Ferritin Vaccines Elicits Neutralizing Antibody Responses against SARS-CoV-2 in Mice. ACS Central Sci 2021, 7 (1), 183–199. https://doi.org/10.1021/acscentsci.0c01405 |
| Zhang, K.; Li, S.; Pintilie, G.; Chmielewski, D.; Schmid, M. F.; Simmons, G.; Jin, J.; Chiu, W. A 3.4-Å Cryo-Electron Microscopy Structure of the Human Coronavirus Spike Trimer Computationally Derived from Vitrified NL63 Virus Particles. Qrb Discov 2020, 1, e11. https://doi.org/10.1017/qrd.2020.16 |
| Zhang, K.; Pintilie, G. D.; Li, S.; Schmid, M. F.; Chiu, W. Resolving Individual Atoms of Protein Complex by Cryo-Electron Microscopy. Cell Res 2020, 30 (12), 1136–1139. https://doi.org/10.1038/s41422-020-00432-2 |
| Roh, S.-H.; Shekhar, M.; Pintilie, G.; Chipot, C.; Wilkens, S.; Singharoy, A.; Chiu, W. Cryo-EM and MD Infer Water-Mediated Proton Transport and Autoinhibition Mechanisms of Vo Complex. Sci Adv 2020, 6 (41), eabb9605. https://doi.org/10.1126/sciadv.abb9605 |
| Fromm, S. A.; Lawrence, R. E.; Hurley, J. H. Structural Mechanism for Amino Acid-Dependent Rag GTPase Nucleotide State Switching by SLC38A9. Nat Struct Mol Biol 2020, 27 (11), 1017–1023. https://doi.org/10.1038/s41594-020-0490-9 |
| Fan, C.; Kaiser, J. T.; Rees, D. C. A Structural Framework for Unidirectional Transport by a Bacterial ABC Exporter. Proc National Acad Sci 2020, 117 (32), 19228–19236. https://doi.org/10.1073/pnas.2006526117 |
| Kumar, A.; Basak, S.; Rao, S.; Gicheru, Y.; Mayer, M. L.; Sansom, M. S. P.; Chakrapani, S. Mechanisms of Activation and Desensitization of Full-Length Glycine Receptor in Lipid Nanodiscs. Nat Commun 2020, 11 (1), 3752. https://doi.org/10.1038/s41467-020-17364-5 |
| Partin, A. C.; Zhang, K.; Jeong, B.-C.; Herrell, E.; Li, S.; Chiu, W.; Nam, Y. Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA. Mol Cell 2020, 78 (3), 411-422.e4. https://doi.org/10.1016/j.molcel.2020.02.016 |
| Chiu, W. Stanford-SLAC Cryo-EM Center. Acta Crystal 2020, 76 (a1), a200–a200. https://doi.org/10.1107/s0108767320098025 |
| Chiu, W.; Li, Y. T.; Bushnell, D.; Schmid, M. F.; Skiniotis, G.; Hedman, B. Stanford-SLAC Cryo-EM Center (S2C2). Microsc Microanal 2019, 25 (S2), 2658–2659. https://doi.org/10.1017/s1431927619014028 |
| Buffalo, C. Z.; Stürzel, C. M.; Heusinger, E.; Kmiec, D.; Kirchhoff, F.; Hurley, J. H.; Ren, X. Structural Basis for Tetherin Antagonism as a Barrier to Zoonotic Lentiviral Transmission. Cell Host Microbe 2019, 26 (3), 359-368.e8. https://doi.org/10.1016/j.chom.2019.08.002 |
Theses
| Hoppe, N.W. Adv (2023) Structural Insights into Orphan G Protein-Coupled Receptors: A Focus on GPR161 and Proton-Sensing GPCRs. UCSF. ProQuest ID: Hoppe_UCSF_0034D_12613. Merritt ID: ark:/13030/m57q9stg. Retreived from https://escholarship.org/uc/item/4n65t4w6 |
| Rosenberg, G.M. (2023). Genetic Causes of Amyloid Fibrils and Their Structures. UCLA. ProQuest ID: Rosenberg_ucla_0031D_22088. Merritt ID: ark:/13030/m5191m86. Retrieved from https://escholarship.org/uc/item/914702p |
