May 2023 CryoEM Image Processing Workshop

Workshop & Lecture Archive

2024 Single-Particle Cryo-EM Image Processing Workshop
In-Person/Virtual Hybrid Workshop September 16-18, 2024
MONDAY, SEPTEMBER 16, 2024 
(Zoom Recording - Passcode d+Q4ksJY) (Ali's SLIDES) (Michael's SLIDES) (Rich's SLIDES)
CryoSPARC; Structure Validation
TIMETITLESPEAKER
8:30 AM - 8:45 AMSession IntroductionWah Chiu
8:45 AM - 10:15 AMCryo-EM Processing FundamentalsCryoSPARC Team: Richard Posert; 
Michael McLean; Ali Punjani
10:15 AM - 10:30 AMBreak 
10:30 AM - 12:00 PMData Processing Case Study ICryoSPARC Team
12:00 PM - 1:00 PMLunch 
1:00 PM - 2:30 PMData Processing Case Study IICryoSPARC Team
2:30 PM - 2:45 PMBreak 
2:45 PM - 4:30 PMData Processing Case Study IIICryoSPARC Team
4:30 PM - 5:30 PMAttendee's Lightning TalksSession 1 of 2
6:00 PM - 8:30 PMDinner Quad Lawn
TUESDAY, SEPTEMBER 17, 2024 
(Zoom Recording - Passcode 9Xfs3&n=) (Greg's SLIDES; Greg's ChimeraX Exercise Document)
CryoSPARC; Structure Validation
8:30 AM -10:15 AMData Processing Case Study IVCryoSPARC Team                              
10:15 AM - 10:30 AMBreak 
10:30 AM - 12:00 PMData Processing Case Study VCryoSPARC Team
12:00 PM - 12:20 PMGroup Photo 
12:20 PM - 1:30 pmLunchQuad Lawn`
1:30 PM - 2:30 PMAdditional Features and DevelopmentsCryoSPARC Team
2:30 PM - 3:30 PMCryoEM Structure Validation 
3:30 PM - 3:45 PMBreak 
3:45 PM - 4:30 PMQuestion and Answer SessionCryoSPARC Team
4:30 PM - 5:30 PMAttendee's Lightning TalksSession 2 of 2
5:30 PMEnd of Day 
WEDNESDAY, SEPTEMBER 18, 2024
(Zoom Recording - Passcode: t^&65Pqw) (Ryan's SLIDES)
CryoDRGN; EMAN 2
8:30 AM - 8:35 AMSession IntroductionWah Chiu
8:35 AM - 10:15 AMCryoDRGN - Part IEllen Zhong Lab at Princeton: Michal 
Grzadkowski; Ryan Feathers
10:15 AM - 10:30 AMBreak 
10:30 AM - 12:00 PMCryoDRGN - Part IIMichal Grzadkowski; Ryan Feathers
12:00 PM - 1:00 PMCryoEM Facility Tour (early Tour)
-Bldg. 006, Bldg. 057 Biolab, &
    Control Room
S2C2 Staff: Patrick Mitchell, Lydia 
Joubert, Nathan Burrows
1:00 PM - 2:00 PMLunch 
2:00 PM - 2:05 PMSession IntroductionWah Chiu
2:05 PM - 3:30 PMEMAN 2 Part IMuyuan Chen
3:30 PM - 3:45 PMBreak 
3:45 PM - 4:45 PMEMAN 2 Part IIMuyuan Chen
4:45 PM - 5:00 PMClosing RemarksWah Chiu
5:00 PM - 6:00 PMCryoEM Facility Tour (Late Tour)S2C2 Staff

 

2023 Image Processing Workshop

In-Person/Virtual Workshop | May 17-19, 2023
Materials: Scipion Agenda; Scipion Slide Decks: Course Motivation; Validation Server; Marcos Gragera
SpeakerInstitutionTalk Title/Slide Deck (see above links)Recording(s)

Wednesday, May 17, 2023

Carlos Sorzano
Marcos Cabezudo
CSIC
CSIC
Theory and Practices: Understanding the effects of the low
SNR on the cryoEM images and the ways to reduce bias.
Introduction to the different softwares to be used in these 
sections, namely: SCIPION, Xmipp, Relion, CryoSPARC, 
Cryolo, GCTF, CTFFind4, GAutomatch, and Topaz
Video 1/Video 2/Video 3
  Practical Exercise: Image processing, particle identification,
intial map, angular refinement
Video 1/Video 2/Video3/
Video 4

Thursday, May 18, 2023

Carlos Sorzano
Marcos Cabezudo
CSIC
CSIC
Theory and Practices: Discrete classification, polishing, map
validation
Video 1/Video 2
  Theory and Practices: Off the beaten path - Beyond discrete
classification: Continuous flexibility analysis (Zernikes, 
CryoDrgn, Normal Modes, and GMM+AI)
Video 1/Video 2

Friday, May 19, 2023

Carlos Sorzano
Marcos Cabezudo
CSIC
CSIC
Theory and Practices: Variability characterizationVideo 1
Muyuan ChenSLACGaussian mixture models for single particle refinement and
heterogeneity analysis (EMANs)
Video 1
Greg PinitilieStanfordCryoEM map and model assessmentVideo 1/Video 2
Tom GoddardUCSFChimeraX cryoEM Visualization Hands-on Tutorial looking
at Bacterial ATP Synthase
Video 1

SSRL/LCLS Users' Meeting - CryoEM Workshop

Virtual Workshop Only | September 26, 2022
Workshop Presentations (not including River Xiao) (no PDFs of talks)
SpeakerInstitutionTalk Title/Slide DeckRecording(s)

Monday, September 26, 2022

Peter PetersMaastricht UniversitySample preparation for Single Particle Analysis and
cryo-EM tomography
See above
Chu-Young KimYale UniversityDetection and repair of noncovalent DNA modificationsSee above
Chencheng FanUniversity of MichiganNeutralizing monoclonal antibodies elicted by mosaic
RBD nanoparticles bind conserved sarbecovirus epitope
See above
River XiaoUniversity of TexasStructural studies of giant icosahedral eukaryotic
dsDNA viruses
Not Aavailable
David ChmielewskiStanfordChikungunya virus assembly and budding visualized in
situ using cryoET
See above

S2C2 High Resolution CryoET Image Processing Virtual Workshop

Virtual Workshop Only | March 1-3, 2022
Workshop Materials: DeRosier's Fourier Transform Treastise Booklet
Goddard's Materials Zip folder; Ludtke's Tutorial Zip Folder
SpeakerInstitutionTalk Title/Slide DeckRecording(s)

Tuesday, March 1, 2022

David DeRosierBrandeis UniversityEM Images: what you want vs what you get and whyVideo 1
Frederick SigworthYale UniversityBasics of Image Processing: from the data you have
to the structures you'd like
Video 1
Thomas GoddardUCSFVisualizing proteasomes in tomograms with ChimeraXVideo 1

Wednesday, March 2, 2022

Steven LudtkeBaylor College of
Medicine
CryoET processing toward atomic resolution 
(EMAN2) Part 1, Part 2
Video 1/Video 2/Video 3

Thursday, March 3, 2022

Dimitry TegunovGenentechUsing the WARP-RELION-M pipeline to obtain high-
resolution structures from tilt series
Video 1/Video 2/Video 3

SSRL/LCLS Users' Meeting: CryoEM of Macromolecules and Cells Workshop

Virtual Workshop Only | September 21, 2021
SpeakerInstitutionTalk Title/Slide DeckRecording(s)
Alfonso MondragonNorthwestern 
University
Structural Studies of a Type III-A CRISPR effector compleexNot Available
Seyed TorabiYale UniversityIntegration of biochemical and structural data to investigate 
stabilizing triplex-forming elements in RNA
Video 1
Kalli KappelMIT Broad InstituteAccelerating RNA structure determination with cryo-EM and 
DRRAFTER computational modeling
Video 1
Zhaoming SuSchuan UniversityCryo-EM reveals multiple states in splicing reaction of 
Tetrahymena intron
Video 1
Kaiming ZhangUniversity of S&T
of China
Determination of small RNA-only 3D structures by Cryo-EMVideo 1

S2C2 CryoEM Map Modeling and Validation Workshop

Virtual Workshop Only | September 8-10, 2021
Workshop Material: Computer Setup & Instructions
Q&A Day 1, Day 2, Day 3
SpeakerInstitutionTalk Title/Slide DeckRecording(s)

Wednesday, September 8, 2021

Christopher SchlicksupLBNLPhenix tools for improving maps, and building, refining,
and validating models
Video 1
Tom GoddardUCSFUsing AlphaFold predicted structures in ChimeraX for 
cryoEM modeling
Video 1
Greg PintilieStanfordModel fitting and assessment in CryoEM maps with
Segger, Segfit, and Q-scores
Video 1
Jasmine Young
Justin Flatt
Rutgers
Rutgers
Deposition, Validation, and Biocuration at the wwPDBVideo 1

Thursday, September 9, 2021

Paul EmsleyMRC Laboratory of
Molecular Biology
Coot Tools for Cryo-EM Model-building, Refinement,
Validation, and Analysis
Video 1
Tristan CrollCambridgeInteractive physics-based model building with ISOLDEVideo 1
Maximilian Edich
Jola Mirecka
Universitat Hamburg
STFC UKRI
SecondaryVideo 1
Grzegorz ChojnowskiEMBL, HamburgMacromolecular models automatically built into cryo-
EM maps and how to use them
Video 1
Jane RichardsonDuke UniversityCryoEM model validation, to avoid or correct errors at 
various resolutions
Video 1

Friday, September 10, 2021

Tristan CragnoliniBirbeck CollegeImproving and validating map/model fits with TEMPyVideo 1
Brinda VallatRutgers Virtual Torsion Plots to Validate Coarse RNA
Conformation
Video 1
Andriy KryshtafovychUC DavisModel comparisonsVideo 1
Ardan PatwardhanEMBL-EBICryo-EM validation from the perspective of the EMDB
and EMPIAR archives
Video 1
Abhishek SingharoyArizona State
University
Molecular dynamics flexible fitting extended: structures,
ensembles and free energy of proteins and small 
molecules Part 1, Part 2
Video 1

S2C2 CryoEM Specimen Preparation and Data Collection Workshop

Virtual Workshop Only | March 3-4, 2021
Workshop Materials: Understanding the invisible hands of sample preparation
Unanswered Q&A from Prof. Peter's Lecture
SpeakerInstitutionTalk Title/Slide DeckRecording(s)

Wednesday, March 3, 2021

Peter J. Peters(M4i) Maastricht
University
Understanding the invisible hands of sample preparation
for cryo-EM
Video 1/Video 2/Video 3/
Video 4
Benjamin BammesDirect Electron, LPOptimizing Direct Detector Design for a Range of Cryo-EM
Techniques
Video 1/Video 2/Video 3/
Video 4
Jeffrey LengyelThermofisherThe latest generation technology for CryoEM allowing for
unparalleled scientific breakthroughs
Video 1/Video 2/Video 3/
Video 4

Thursday, March 4, 2021

Christopher RussoLMB, MRCPrinciples of information loss in cryoEM and how 
eliminating specimen movement can improve 
cryomicrographs
Video 1/Video 2/Video3
Sahil GulatiAmetekAdvances in direct electron detection: getting the most 
out of your microscope
Video 1/Video 2/Video 3/
Video 4/Video 5
Yee Ting LiSLACData Management at S2C2Video 1/Video 2
Patrick MitchellSLACS2C2 data collectionVideo 1
  Final Q&AVideo 1, Video 2, Video3

CryoEM In-Residence Training and Public Lecture: Tomgraphy and Volta Phase Plate

Virtual Workshop Only | December 10-11, 2020
Provided Agenda

S2C2 CryoEM CCP-EM Modeling Workshop

Virtual Workshop Only | November 10-13, 2020
Workshop Materials: computing instructions
SpeakerInstitutionTalk Title/Slide DeckRecording(s)

Tuesday, November 10, 2020

Colin PalmerCCP-EMLecture: Intro to workshop and CCP-EMVideo 1
Kevin CowtanUniversity of YorkLecture: Automated model building with Buccaneer & Nautilus
Tutorial: Buccaneer
Video 1
Video 1/Video 2/Video3
Paul EmsleyMRC, LMBLecture: Cryo-EM model building with modern COOT
Tutorial: COOT (Part 1)
Video 1
Video 1

Wednesday, November 11, 2020

Paul EmsleyMRC, LMBTutorial: COOT (Part 2)Video 1/Video 2
Maya TopfISMB, BirkbeckLecture: Flexible fitting of Atomic ModelsVideo 1
Tristan CragnoliniISMB, BirkbeckTutorial: Flex-EM (Part 1)Video 1
Aaron SweeneyUniversity College
of London
Tutorial: Flex-EM (Part 2)Video 1
Andrea ThornUniversity of 
Wurzburg
Lecture: Finding secondary structure in cryo-EM maps: 
HARUSPEX
Video 1

Thursday, November 12, 2020

Rob NichollsMRC, LMBLecture: Refinement against cryo-EM Maps
Tutorial: Refmac (Part 1)
Tutorial: Refmac (Part 2)
Video 1
Video 2
Video 3
Ardan PatwardhanEMBL-EBILecture: PDB, EMDB, & Empiar: Public archiving of cryoEM
data
Video 1
Arjen JakobiTU DelftLecture: Map sharpening tools for cryo-EM density maps
Tutorial: LocScale
Video 1
Video 2

Friday, November 13, 2020

Agnel JosephCCP-EMLecture: Validation of cryo-EM maps and Models
Lecture: Model Validation
Tutorial: Model Validation
Video 1
Video 2
Video 3
All Speakers General Q&A Session 

SSRL/LCLS Users' Meeting: Cryo-EM of COVID-related Macromolecules

Virtual Workshop Only | September 28, 2020
SpeakerInstitutionTalk Title/Slide DeckRecording(s)
David VeeslerUniversity of WashingtonStructural Studies of the SARS-CoV Spike GlycoproteinVideo 1/Video 2
David StuartOxford University/
Diamond Light Source
The use of cryoEM structural information to advance
SARS2-CoV-2 biology and therapy
Video 1/Video 2
Goran KokicMax Planck InstituteStructure of replicating SARS-COV-2 polymeraseVideo 1
Erica O. Saphire
Adrian Enriquez
La Jolla InstituteAntibodies against emerging viral diseaseVideo 1/Q&A
Rhiju Das
Ivan Zheludev
StanfordCryoEM of previously unsolvable RNA structures 
including coronavirus genome elements
Video 1/Q&A
Stephan WilkensSUNY Upsate Medical
University
Targeting Vacuolar H+-ATPase for Antivral TherapyVideo 1/Video 2/Video3

Fundamentals of Single Particle CryoEM Workshop

Virtual Workshop Only | August 11 - 12, 2020
SpeakerInstitutionTalk Title/Slide DeckRecording(s)

Tuesday, August 11, 2020

Mark HerzikUC San DiegoSample preparation for cryo-EMVideo 1/Video 2
David DeRosierBrandeis UniversityImage contrast theory and reconstructionVideo 1/Video 2
Alexis RohouGenentechPrinciples of image processingVideo 1/Video 2
Andres LeschzinerUC San DiegoDetermining structures of challenging
samples Part 1/Part 2/Part 3
Video 1/Video 2

Wednesday, August 12, 2020

Dmity LyumkisSalk InstituteDealing with resolution anisotropy and
considerations for high resolution structure
determination
Video 1/Video 2/Video 3
Wah ChiuStanford/SLACAtomic modeling and the importance of validationVideo 1/Video 2
Ali PunjaniStructural BiotechnologyCryoSPARC demonstration/tutorial (see below June
workshop for slides)
Video 1/Video 2/Video 3/
Video 4

S2C2 CryoEM Image Processing Workshop: cryoSPARC

Virtual Workshop Only | June 10 - 12, 2020
Workshop Materials: user interface overview; cryoSPARC Instructions Day 1/Day 2/ Q& A Day 1, Day 2, Day 3
SpeakerInstitutionTalk Title/Slide DeckRecording(s)

Wednesday, June 10, 2020

David DeRosierBrandeis UniversityIf only...Video 1/Video 2/Video 3/
Ali PunjabiStructural BiotechnologyCryoSPARC (AM) User Interface overview/
Day 1 CryoSPARC Instructions
Video 1/Video 2/Video 3/
Video 4/Video 5/Video 6
Ali PunjabiStructural BiotechnologyCryoSPARC (PM)Video 7/Video 8/Video 9/
Video 10/Video 11/Video 12

Thursday, June 11, 2020

Ali PunjabiStructural BiotechnologyCryoSPARC (AM)Video 1/Video 2/Video 3/
Video 4/Video 5/Video 6/
Video 7/Video 8
Ali PunjabiStructural BiotechnologyCryoSPARC (PM)Video 9/Video 10/Video 11/
Video 12
Stephan ArulthasanStructural BiotechnologyCryoSPARC architecture & managmentVideo 1
Ali PunjabiStructural BiotechnologyCryoSPARC (PM)Video 13/Video 14/Video 15/
Video 16

Friday, June 12, 2020

Greg PintilieStanford UniversityCryoEM map resolution assessment,
validation, visualization, and segmentation
Video 1/Video 2/Video 3
Cathy LawsonRutgersEMDataResource: structure data archiving,
validation challenges
Video 1/Video 2

Basics of Sample Preparations & Detection Technologies for Cryo-Electron Microscopy

Virtual Lecture Only | May 29, 2020
SpeakerInstitutionTalk Title/Slide DeckRecording(s)
Yifan ChengHHMI UC San FranciscoBasics of sample preparationNot Available
Christopher BoothAmtekDetection technologies for cryo-electron microscopyVideo 1/Video 2/
Video 3/Video 4/
Video 5/ Video 6

General Principles of Phase Contract and Phase Plates & Single-Particle Grid Preparation

Virtual Lecture Only | April 20, 2020
SpeakerInstitutionTalk Title/Slide DeckRecording(s)
Robert GlaeserLBNLGeneral Principles of phase contrast and phase plates
Single-particle grid preparation
Video 1
Video 2
Specimen Prep and Data Collection Workshop
In-Person Workshop | March 2020 (CANCELLED DUE TO COVID)

S2C2 CryoEM Modeling Workshop: ChimeraX & Phenix

In-Person Workshop | January 15-17, 2020
SpeakerInstitutionTalk Title/Slide DeckRecording

Wednesday, January 15, 2020

Tom Goddard
Elaine Meng
Eric Pettersen
UCSF
UCSF
UCSF
ChimeraX-Visualization of cryoEM maps and modelsNot Available
Greg PintilieStanfordSegmentation, rigid body and flexible fittingNot Available
Tom Goddard
Elaine Meng
Eric Pettersen
UCSF
UCSF
UCSF
Refinement of cryoEM maps with IsoldeNot Available
Tom GoddardUCSFPublication-quality images and moviesNot Available

Thursday, January 16, 2020

Paul AdamsLBNLPhenix Introduction (The entire day is include in one video)
Atomic models from cryo-EM data
Video 1
Pavel AfonineLBNLStructure Refinementsee above
Paul AdamsLBNLModel validation for cryo-EMsee above
Pavel AfonineLBNLStructure refinement and validation practicalsee above
Nigel MoriartyLBNLLigandssee above

Friday, January 17, 2020

Greg PintilieStanfordAssessment of high resolution cryoEM featuresVideo 1
Helen BermanRutgersValidation of biological sctructuresNot available
Cathy LawsonRutgersEM structure archives, validation and challengessee above

S2C2 CryoEM Image Processing Workshop: SCIPION

In-Person/Zoom | November 18-20, 2019
SpeakerInstitutionTalk Title/Slide DeckRecording(s)

Monday, November 18, 2019

David DeRosier Brandeis UniversityGeneral Principles of cryoEM 3D reconstructionsVideo 1/Video 2/Video 3
Jose Maria CarazoCNB-CSICBasic image processing for EM (course material)Video 1/Video 2/Video 3

Carlos Sanchez-

Sorzano

CNB-CSICImage processing principles

Video 1/Video 2/Video 3/

Video 4/Video 5

Tuesday, November 19, 2019

Yiorgio SkiniotisStanford

Dealing with heterogeneity in single-particle

data/examples

Not Available

Wednesday, November 20, 2019

Greg PintilieStanfordModel building and map validationNot Available

S2C2 CryoEM Training for Beginners Workshop

In-Person Workshop | September 11 - 13, 2019
SpeakerInstitutionTalk Title/Slide DeckRecording

Wednesday, September 11, 2019

Juegen PlitzkoMax Planck InsituteEM BasicsNot Available
Yiorgio SkiniotisStanfordFreezing BasicsNot Available

Thursday, September 12, 2019

Christopher BoothGatanCameraNot Available
Yee Ting LiSLACeLogbook and data transferNot Available
Robert GlaeserLBNLRadiation damageNot Available

Friday, September 13, 2019

Zoltan MetlagelFEIEPU and phase plateNot Available
Corey HeckselSLACData collection (Serial EM)Not Available

S2C2 CryoEM Modeling Workshop: ChimeraX & Phenix

In-Person Workshop | July 10-12, 2019

Materials: properly weighted map t20s | model validation tutorial | model optimization tutorial

SpeakerInstitutionTalk Title/Slide DeckRecording

Wednesday, July 10, 2019

Tom Goddard

Elaine Meng

Eric Pettersen

UCSF

UCSF

UCSF

ChimeraX - visualization of cryoEM Maps and ModelsNot Available
Greg PintilieStanfordRigid body and flexible fittingNot Available

Thursday, July 11, 2019

Paul AdamsLBNLPhenix introductionNot Available
Paul AdamsLBNLAutomated model docking and buildingNot Available
Pavel AfonineLBNLPhenix tools for cryoEM: refinement and validationNot Available
Paul AdamsLBNLModel validation for cryo-EMNot Available
Nigel MoriartyLBNLRestraints in PhenixNot Available

Friday, July 12, 2019

Corey Hyrc

Baylor College of 

Medicine

Accurate representation of cryoEM StructuresNot Available
Greg PintilieStanfordAssessment of high resolution of cryoEM FeaturesNot Available
Michael RoberstonStanfordCryoEM structure and ligand modelingNot available
Cathy LawsonRutgersCryoEM archives, challenges and validationNot Available

S2C2 CryoEM Image Processing Workshop: SCIPION

In-Person Workshop | November 18-20, 2019
SpeakerInstitutionTalk Title/Slide DeckRecording

Monday, November 18-20, 2019

David DeRosierBrandeis UniversityGeneral principles of CryoEM image reconstructionNot Available
Yee Ting LiSLAC

Data Acquisition and pre-processing software logging

on and computers basics

Not Available
Cornelius GatiSLACImage Processing with Relion - IntroductionNot Available

Tuesday, November 19, 2019

Cornelius GatiSLACPractical 2 & 3Not Available

Wednesday, November 20, 2019

Greg Pintilie

Wah Chiu

Stanford

Stanford/SLAC

Map visualization and validationNot Available
Cathy LawsonRutgers UniversityCryo-EM map challenge, map deposition and archivingNot Available
Yiorgio SkiniotisStanford

Dealing with heterogeneity in single-particle cryoEM

data

Not Available
Cornelius GatiSLACDifferent image processing software packagesNot Available

S2C2 CryoEM Training for Begineers Workshop

In-Person Workshop | September 11-13, 2019
SpeakerInstitutionTalk Title/Slide DeckRecording

Wednesday, September 11, 2019

Juergen PlitzkoMax Planck InstituteEM BasicsNot Available
Yiorgo SkiniotisStanfordFreezing BasicsNot Available

Thursday, September 12, 2019

Chris BoothGatanCameraNot Available
Yee-Ting LiSLACeLogbook and data transferNot Available
Robert GlaeserLBNLRadiation DamageNot Available

Friday, September 13, 2019

Zoltan MetlagelThermofisherEPU and Phase PlateNot Available
Corey HeckselSLACData Collection (Serial EM)Not Available

S2C2 Modeling Workshop: ChimeraX & Phenix

In-Person Workshop | July 10-12, 2019
SpeakerInstitutionTalk Title/Slide DeckRecording

Wednesday, July 10, 2019

Tom Ferrin

Tom Goddard

UCSFChimeraX  - Visualization of CryoEM maps & modelsNot Available
Greg PintilieStanfordRigid Body and Flexible FittingNot Available
Michael SchmidSLACHands on VR to Visualize CryoEM StructuresNot Available

Thursday, July 11, 2019

Paul AdamsLBNLPhenix Intro/Automated Model Docking & BuildingNot Available
Pavel AfonineLBNLStructure RefinementNot Available
Dorothee LiebschnerLBNLValidation and RefinementNot Available
Paul AdamsLBNL

Intro to Phenix Cryo-EM Tools Practical/Automated

Model Building

Not Available
Pavel AfonineLBNLStructure Refinement PracticalNot Available
Dorothee LiebschnerLBNLValidation and Refinement PracticalNot Available

Friday, July 12, 2019

Corey Hyrc

Baylor College of

Medicine

Accurate Representation of CryoEM StructuresNot Available
Greg PintilieStanfordAssessment of High Resolution CryoEM FeaturesNot Available
Mike RobertsonStanfordCryoEM Structure Ligand ModelingNot Available
Cathy LawsonRutgersCryoEM Structural ValidationNot Available

S2C2 Cryo-EM Image Processing Workshop

In-Person Workshop | March 11-13, 2019
SpeakerInstitutionTalk Title/Slide DeckRecording

Monday, March 11, 2019

David DeRosierBrandeis UniversityGeneral Principles of CryoEM Image ReconstructionNot Available
Yee-Ting LiSLAC

Data Acquistion and pre-processing software, logging on and 

computer basics

Not Available
Cornelius GatiSLAC

Image Processing with Relion Intro/Practical 1: Images, 

MotionCor, CTF

Not Available

Tuesday, March 12, 2019

Cornelius GatiSLAC

Practical 2: 2D/3D Classification/Practical 3: 3D Refinement 

& Map Improvement

Not Available

Wednesday, March 13, 2019

Greg Pintilie

Wah Chiu

SLACMap Visualization and ValidationNot Available
Cathy LawsonRutgersMap Challenge and Map Deposition and ArchivalNot Available
Yiorgio SkiniotisStanfordDealing with Heterogeneity in single-particle cryoEM dataNot Available
Kaiming ZhangStanfordImage Processing of Heterogeneous Particle ImagesNot Available
Bart BuijsseThermofisherUpdate on Volta Phase PlateNot Available
2019 Workshop/Lectures

S2C2 Cryo-EM Training for Beginners Workshop

In-Person Workshop | January 22-25, 2019

SpeakerInstitutionTalk Title/Slide DeckRecording(s)

Tuesday, January 22, 2019

Yiorgo SkiniotisStanfordCryo-Specimen PreparationNot Available
Wah ChiuStanford/SLACRadiation Damage in cryoEMNot Available
Christopher BoothGatanDetection technologies for cryo-electron microscopyNot Available

Wednesday, January 23, 2019

Juergen Plitzko

Max Planck

Institute

TEM Basics and column alignmentNot Available
David BushnellStanford/SLACLow dose imaging via serial EM and EPUNot Available
Wah ChiuStanford/SLACImage contrast theory in CryoEMNot Available

Thursday, January 24, 2019

John SpearThermofisherKrios and Talos alignmentNot Available
Yee Ting LiSLACeLogbook, data collection monitoring & pre-processingNot Available