Topological crossing in the misfolded Tetrahymena ribozyme resolved by cryo-EM

TheTetrahymenagroup I intron has been a key system in the understanding of RNAfolding and misfolding. The molecule folds into a long-lived misfolded intermediate(M)in vitro, which has been known to form extensive native-like secondary and tertiarystructures but is separated by an unknown kinetic barrier from the native state (N). more...